Over several decades, poly (lactic-co-glycolic acid) (PLGA) have been widely\nused as Micro- and Nano-carriers of therapeutic agents for drug delivery applications.\nHowever, encapsulation process of therapeutic agents into PLGA\nNanoparticles (NPs) necessitates a defined step to understand the effects and\ninteractions of parameters involved in production process. In pharmaceutics\nformulations, compared to one factor at a time (OFAT) approach, statistical\ndesign of experiments (DOE) supersedes OFAT approach due to limited\nnumber of experiments required to investigate effects and interactions of a\nprocess parameters. The major objectives of the present study were to: 1) prepare\nand understand the effect of selected formulation parameters on particles\nsize and drug recovery of PLGA NPs encapsulating Ciprofloxacin Hydrochloride\n(Cip-HCl) using a fractional factorial design (FFD) as a DOE approach;\n2) understand the in -vitro release of Cip-HCl from PLGA NPs. Cip-HCl\nloaded PLGA were prepared by W1/O/W2 double emulsion solvent evaporation\n(DESE) method using poly-vinyl alcohol as a stabilizer. The Sizes of NPs\nwere within 202 nm to 530 nm and percentage Cip-HCl recovered from dried\nNPs were within 1.7% w/w to 15.7% w/w. Increasing concentrations of PLGA\nand Cip-HCl was observed to increase NPs size. Increasing PVA concentration\nwas observed to either reduce or increase NPs size. Increasing PLGA\nconcentration was observed to increase the amount of Cip-HCl recovered.\nWithin 1 - 24 hours, optimized formulations shows a controlled release of\nCip-HCl from PLGA NPs.
Loading....